Sage ELF—可将DNA样品按大小回收成12个组份，还可以用于回收蛋白
• Sage Science 公司
位于美国马萨诸塞州，2010年成立，专注于核酸领域的样品制备
全球超过3000家客户的认可，国内装机数量400台左右
2万篇文献中应用，包括众多Nature、 Science、 Cell系列的文章
DNA片段筛选回收领域的行业金标准
• Sage ELF 全自动DNA片段连续回收系统
自动化完成核酸片段大小范围的回收（size-selection）
用于从单一样本构建多个不同长度的文库，或者是RNA iso-form / splice variant detection转录异构体的建库、或是珍贵样本的建库等
自带脉冲场模块，实现100bp－40kb范围内的DNA样本分离回收效果；也可以实现蛋白回收
技术原理
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加利福尼亚大学、耶鲁大学、哈佛大学等多个科研单位，联合开展研究绘制出了人类泛基因组参考图谱。相关成果在国际权威期刊《Nature》杂志发表。 文章中Sage ELF用于HiFi文库片段筛选（Liao, Wen-Wei, et al. "A draft human pangenome reference." Nature 617.7960 (2023): 312-324.）
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发表文献
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1.Kratochwil, Claudius F., et al. "An intronic transposon insertion associates with a trans-species color polymorphism in Midas cichlid fishes." Nature Communications 13.1 (2022): 296. 2.Bickhart, Derek M., et al. "Generating lineage-resolved, complete metagenome-assembled genomes from complex microbial communities." Nature biotechnology 40.5 (2022): 711-719. 3.Kuhl, Heiner, et al. "Equilibrated evolution of the mixed auto-/allopolyploid haplotype-resolved genome of the invasive hexaploid Prussian carp." Nature communications 13.1 (2022): 4092. 4.Jarvis, Erich D., et al. "Semi-automated assembly of high-quality diploid human reference genomes." Nature (2022): 1-13. 5.Cerca, José, et al. "The genomic basis of the plant island syndrome in Darwin’s giant daisies." Nature communications 13.1 (2022): 3729. 6.Zou, Changsong, et al. "The genome of broomcorn millet." Nature communications 10.1 (2019): 436. 7.Kolmogorov, Mikhail, et al. "metaFlye: scalable long-read metagenome assembly using repeat graphs." Nature Methods 17.11 (2020): 1103-1110. 8.Wenger, Aaron M., et al. "Accurate circular consensus long-read sequencing improves variant detection and assembly of a human genome." Nature biotechnology 37.10 (2019): 1155-1162. 9.Wu, Zhikun, et al. "Structural variants in the Chinese population and their impact on phenotypes, diseases and population adaptation." Nature Communications 12.1 (2021): 6501. 10.Sun, Yu H., et al. "Single-molecule long-read sequencing reveals a conserved intact long RNA profile in sperm." Nature communications 12.1 (2021): 1361. |
