如何进行长片段基因组DNA质控？—Pippin Pulse脉冲场电泳系统
随着三代测序技术的发展和普及，全基因组测序技术也取得了长足发展。基因组DNA文库的制备质量直接影响测序结果，因而基因组DNA的质控越来越受到重视。而实验室常规的琼脂糖凝胶电泳只能分离20kb以下的DNA分子，当DNA分子大于20kb时，就无法实现有效分离。
针对超长DNA片段的分离和质控，美国Sage Science公司拥有超高性价比的Pippin Pulse脉冲场电泳系统。Pippin Pulse使用简单，无专用耗材，自带预设程序，能很好的电泳分离0.5Kb到430Kb的基因组DNA片段。Pippin Pulse具有一个简单的软件界面，自带6种预设程序，可以直接分离多种不同大小范围DNA长片段，此外，软件还支持自定义模式，用户可以自编程分离不同范围的DNA片段。Pippin Pulse包括电源、电泳槽、操控仪器的电脑和用户手册，无特殊耗材，使用成本低，经济耐用。
Pippin Pulse脉冲场电泳系统
Pippin Pulse脉冲场电泳系统预设程序
Pippin Pulse脉冲场电泳系统结果图示
Pippin Pulse脉冲场电泳系统应用案例（部分展示）
1、Pippin Pulse 与PacBio三代测序仪联用，控制文库片段分布，充分发挥长片段测序优势
| • Takeuchi, Tadashi, et al. "Fatty acid overproduction by gut commensal microbiota exacerbates obesity." Cell Metabolism (2023). |
| • Hadzhiev, Yavor, et al. "The miR-430 locus with extreme promoter density forms a transcription body during the minor wave of zygotic genome activation." Developmental Cell 58.2 (2023): 155-170. |
| • Kurokochi, Hiroyuki, et al. "Telomere-to-telomere genome assembly of matsutake (Tricholoma matsutake)." DNA Research 30.3 (2023): dsad006. |
| • Hixson, Kim K., et al. "Annotated genome sequence of a fast-growing diploid clone of red alder (Alnus rubra Bong.)." G3: Genes, Genomes, Genetics (2023): jkad060. |
| • Field, Matt A., et al. "The Australian dingo is an early offshoot of modern breed dogs." Science Advances (2022): eabm5944. |
| • Pootakham, Wirulda, et al. "A Chromosome-Scale Genome Assembly of Mitragyna speciosa (Kratom) and the Assessment of Its Genetic Diversity in Thailand." Biology 11.10 (2022): 1492. |
| • Rahman, M. Julhasur, et al. "LINE-1 retrotransposons facilitate horizontal gene transfer into poxviruses." Elife 11 (2022): e63327. |
| • Zhao, Qing, et al. "The reference genome sequence of Scutellaria baicalensis provides insights into the evolution of wogonin biosynthesis." Molecular plant 12.7 (2019): 935-950. |
2、 Pippin Pulse 与 Nanopore三代测序仪联用，质控更长、更高质量的DNA，进一步提升测序长度
| • Long, Meng, et al. "Comparative genomic analysis provides insights into taxonomy and temperature adaption of Aeromonas salmonicida." Journal of Fish Diseases 46.5 (2023): 545-561. |
| • Keraite, Ieva, et al. "A method for multiplexed full-length single-molecule sequencing of the human mitochondrial genome." Nature Communications 13.1 (2022): 5902. |
| • Ong, Chian Teng, et al. "Adaptive sampling during sequencing reveals the origins of the bovine reproductive tract microbiome across reproductive stages and sexes." Scientific reports 12.1 (2022): 15075. |
| • Wunderlich, Stephanie, et al. "Targeted biallelic integration of an inducible Caspase 9 suicide gene in iPSCs for safer therapies." Molecular Therapy-Methods & Clinical Development 26 (2022): 84-94.v |
| • Russo, Alessia, et al. "Low-input high-molecular-weight DNA extraction for long-read sequencing from plants of diverse families." Frontiers in Plant Science 13 (2022): 1494. |
| • Player, Robert A., et al. "A novel Canis lupus familiaris reference genome improves variant resolution for use in breed-specific GWAS." Life science alliance 4.4 (2021). |
| • Young, Amy E., et al. "Genomic and phenotypic analyses of six offspring of a genome-edited hornless bull." Nature biotechnology 38.2 (2020): 225-232. |
| • Belser, Caroline, et al. "Chromosome-scale assemblies of plant genomes using nanopore long reads and optical maps." Nature plants 4.11 (2018): 879-887. |
3、Pippin Pulse 与 Bionano分子光谱仪联用，确保HMW DNA，保证分析结果准确性
| • Secomandi, Simona, et al. "A chromosome-level reference genome and pangenome for barn swallow population genomics." Cell Reports 42.1 (2023): 111992. |
| • Dahn, Hollis A., et al. "Benchmarking ultra-high molecular weight DNA preservation methods for long-read and long-range sequencing." GigaScience 11 (2022). |
| • Palmada-Flores M, Orkin J D, Haase B, et al. A high-quality, long-read genome assembly of the endangered ring-tailed lemur (Lemur catta)[J]. GigaScience, 2022, 11. |
| • Canaguier A, Guilbaud R, Denis E, et al. Oxford Nanopore and Bionano Genomics technologies evaluation for plant structural variation detection[J]. BMC genomics, 2022, 23(1): 1-17. |
| • Shukla H, Suryamohan K, Khan A, et al. Near-chromosomal de novo assembly of Bengal tiger genome reveals genetic hallmarks of apex-predation[J]. bioRxiv, 2022. |
| • Rhie, Arang, et al. "Towards complete and error-free genome assemblies of all vertebrate species." Nature 592.7856 (2021): 737-746. |
| • Belser, Caroline, et al. “Telomere-to-telomere gapless chromosomes of banana using nanopore sequencing.” Communications biology 4.1 (2021): 1047. |
| • Zheng, Yuting, et al. "Rapid prenatal diagnosis of Facioscapulohumeral Muscular Dystrophy 1 by combined Bionano optical mapping and karyomapping." Prenatal Diagnosis 40.3 (2020): 317-323. |
环亚生物科技有限公司作为美国Sage Science公司Pippin Pulse脉冲场电泳系统在中国区的独家代理商，自2011年以来将Sage Science的产品线引入国内，一直为国内用户提供专业的全自动核酸片段回收系统的安装测试、应用培训，技术支持与售后维护工作，赢得客户的一致好评与信任。环亚生物科技有限公司将一如既往地支持越来越多的Sage Science用户。
▼更多 Pippin Pulse 产品详情，请联系我们▼
环亚生物科技有限公司
地址：上海市闵行区友东路358号闵欣大厦1号楼701/703/705室
电话：021-54583565
邮箱：info@apgbio.com
网址：www.apgbio.com
▼更多精彩内容，请扫码关注我们▼
