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zenCELL owl活细胞监测用于生物材料的研究
 

        生物材料(Biomaterial)的概念源于上世纪90年代,研究范畴涉及材料科学、生物学、解剖学、病理学等多学科的相互交叉和渗透。生物材料的种类繁多,分类方法也很多,应用极为广泛,比如有机材料的一类高分子生物聚合物,因其独特属性多应用于仿生学、临床医学等研究,并逐步实现工程技术的转化。目前,生物聚合粒子的形状多为纤维状或球形,可有效应用于医学领域,如用于组织工程的仿生纤维或可注射的胶囊。
        该文章发表于Applied Materials Today当代应用材料(季刊,聚焦于交叉学科、新材料的应用前沿)的最新一期(2021.09)。研究者们通过调整静电纺丝参数,结合纺前原液的结构着色,合成了一种全新形态的高分子生物聚合材料,融合现有独立形态的纤维状和球形的结构特点,呈现哑铃形的聚ε-己内酯(PCL)微粒子 。为探究哑铃形的聚ε-己内酯(PCL)微粒是否可以模拟细胞外间质(extracellular matrix,ECM)的生物特性,文章中建立U87细胞(人神经胶质瘤细胞)模型进行扫描电镜观察和活细胞动态监测实验。两种实验方法得到一致性结果,即对比传统的PCL纤维结构,该合成微粒子可模拟ECM并有效增强细胞信号转导--细胞更强粘附于微粒子上,进而形成增殖的哑铃簇。
 
        活细胞动态监测实验由德国 InnoME 的 zenCELL owl 活细胞动态成像及分析系统(以下简称zenCELL owl)完成。在该实验中,U87细胞以30,000个细胞每孔的接种密度铺在24孔板内,孔板板底进行胶质包被和无包被处理,同时细胞培养基内加入/不加入密度为1 mg/ml的合成微粒子。将U87细胞模型置于细胞培养箱内,zenCELL owl设置监测间隔17分钟,监测总时长3天,对不同实验组的U87细胞模型进行长时间的动态监测,获取其动态变化过程中的图像、视频和数据。(图1)
 
图1  zenCELL owl设置条件
 
        图2 A-H生动地展示了同一个位置点,U87细胞模型在24小时监测下的动态变化。在监测过程中,U87细胞会附着在呈哑铃形的PCL颗粒上,进一步迁移聚集形成哑铃簇状结构,并继续进行增殖。 
 
图2  U87细胞在含有呈哑铃形PCL颗粒/胶质包被的24孔板培养48小时的细胞图片
(黑色物质为呈哑铃形PCL颗粒)
 
       如下视频1、视频2源于该文章的supplement附录部分,真实反映了U87细胞模型的动态变化过程。
 
视频1  U87细胞在含有呈哑铃形PCL颗粒/胶质包被的24孔板培养72小时的细胞视频
      
视频2  U87细胞在胶质包被的24孔板培养72小时的细胞视频
 
        这些结果清楚地揭示了哑铃形的聚ε-己内酯(PCL)微粒子,其结构可有效地模拟细胞外间质的生物特性,在组织工程领域具有非凡潜力,如注射、生物制造、化妆品和医用填充等,不断开拓新材料的应用前景。
 
       德国InnoME公司自主研发和生产的zenCELL owl活细胞动态成像及分析系统,基于非标记、非侵入方法,原位记录细胞实时生长和定量的分析数据。zenCELL owl体积小巧,耐温耐湿,可在细胞培养箱内长时间连续工作;通过24个显微成像镜头,对24个视野进行连续的动态监测和图像获取。zenCELL owl为用户提供高质量图像、视频、统计学曲线和定量数据,实现细胞增殖/增殖抑制、细胞培养质控/培养优化、迁移/侵袭等诸多活细胞动态过程的监测。
 
       环亚生物,生命科学产品全国性代理商,不断把国外顶级的创新产品引入中国。环亚生物具备完善的公司运营、产品管理、营销、售前技术支持和售后维修体系。环亚生物作为zenCELL owl大中华区独家代理商,负责zenCELL owl产品大中华区的营销和售后支持。
 
       更多资讯请访问文献全文链接:
https://www.sciencedirect.com/science/article/pii/S2352940721001621?via%3Dihub
 

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