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Ocus切片扫描在胶质瘤肿瘤微环境研究中的高效应用
 
胶质瘤(Glioma),是一类最常见的中枢神经系统原发性恶性肿瘤,现有的治疗手段包括手术切除、放疗和化疗。同其他肿瘤类型一样,胶质瘤因先天遗传因素和环境致癌因素互作导致,具体因果关系目前不明确。近年来,越来越多的研究表明肿瘤微环境(tumor microenvironment ,TME)在肿瘤进展中具有重要性和复杂性。
 

2021年4月发表在《Frontiers in Oncology》的这篇研究文章,来自复旦大学附属华山医院神经外科。该团队应用生信技术,识别、筛选出参与胶质瘤TEM调控的核心基因,继而在临床病例中,利用免疫组化染色的方法评分计算这些基因的表达水平。文章核心探讨了神经胶质瘤TEM的免疫和基质组分及其相关关键基因的表达,通过生物信息联合分析并预测MYD88为关键基因(髓样分化初级反应蛋白88 (Myeloid differentiation primary response protein 88, MYD88主要位于血浆中)。同时,MYD88可做为胶质瘤TME的潜在预后基因和免疫标记,并与较高的M2巨噬细胞百分比显著相关。这些结果在CGGA数据库和华山医院31例临床病例中得到验证。
 
实验过程中,31例临床病例的免疫组化(IHC方法)切片,使用Ocus切片扫描显微成像系统(芬兰Grundium)进行数字化扫描和图像保存及导出,并根据国际标准进行评估分级(图1)。
 
图1(C–J) CD68 and CD163 IHC staining image of adjacent slides in clinical glioma patients with WHO grade II/IDH1(+), WHO grade II/IDH1 (–), WHO grade III/IDH1 (–) and WHO grade IV/IDH1 (–) pathology results separately.

同时 Ocus切片扫描显微成像系统(芬兰Grundium)又对MYD88表达的组织染色切片进行数字化扫描和图像保存及导出,根据国际标准进行评估分级(图2)。
 
图2 (C–F) IHC image of example glioma patients with WHO grade II/IDH1(+), WHO grade II/IDH1 (–), WHO grade III/IDH1 (–) and WHO grade IV/IDH1 (–) pathology results separately.

最终,该文章得出结论,MYD88对TIICs异常以及M2型巨噬细胞影响的神经胶质瘤患者,具有预后评估价值。
 
芬兰Grundium公司自主研发和生产了全球首款便携式数字病理扫描显微成像系统--Ocus。该设备采用智能云技术,用户可在任何有网络的地方远程控制和访问其内部的高清图片,并支持多人实时同屏阅片。Ocus体积小巧,可装入个人行李箱中,并支持移动充电;Ocus具备强大的内嵌式电脑,不需外配服务器和电脑,可通过移动终端(手机、平板、笔记本电脑)的网页浏览器在地球上任何有网络的地方进行远程数据访问和高清图片共享。
 
环亚生物,生命科学产品全国性代理商,不断把国外顶级的创新产品引入中国。环亚生物具备完善的公司运营、产品管理、营销、售前技术支持和售后维修体系。环亚生物作为Grundium大中华区独家代理商,负责Grundium产品大中华区的营销和售后支持。  
 
更多资讯请访问文献全文链接:
https://www.frontiersin.org/articles/10.3389/fonc.2021.654388/full
 
 
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