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zenCELL活细胞监测用于草履虫模型进行水质研究



水—自然界的生命之源!
 
        由于人类对金属开采、冶金的日益频繁,以及水的过度浪费,水的重金属污染和严重超标已成为全球面临的严峻问题。汞金属,一种即使低浓度接触仍会危及生命的重金属,被普遍认为是重金属污染之首。据多名科学家于2018年至2020年发表的相关文章表明,在实验室环境下,吸附处理技术可以有效的去除水溶性汞金属,为水质改良提供有力的研究依据。
 
 
        该文章,发表于环境类优质期刊Chemospherre最新一期(2021.07)。研究者们充分利于了水中模式生物—草履虫,研究混合气凝胶(一类高效吸附剂)对水溶性汞金属的原位吸附。
       
        草履虫,单细胞原生动物,水生纤毛虫类之一,同时也是食物链的最底端。其体型小、生命周期短、对环境变化高敏感,可作为水质污染的有效指标。在该实验中,草履虫水系培养,分别加入浓度为0 /125/250/500/750/1000/1500 ug/ul的水溶性汞作为对照组,以及加入硅胶-明胶混合气凝胶(24wt%明胶含量)作为实验组,通过zenCELL owl活细胞动态成像及分析系统(以下简称zenCELL owl)实时记录并监测整个吸附过程(如图a)。

图a: 实验流程和结果的图形概要
左侧为zenCELL owl实时监测草履虫在水系培养中的状态变化(含有各种浓度的水溶性汞,不添加/添加混合气凝胶),蓝色物质-水溶性汞,红色物质-混合气凝胶;右侧为监测后数据结果的统计和分析
 
        zenCELL owl具体设置为间隔5分钟,总时长24小时,不间断、实时地对草履虫培养水系进行图像获取(如图b)。为了有效评估草履虫运动能力及存活能力,ImageJ软件对zenCELL owl获取的一系列原始图片沿时间进度、逐一将前一个时间点的图片作为像素背景扣除,图片像素差异值转换为草履虫细胞的存活数量。继而,IBM®   SPSS® Statistics v22.0 软件中的未配对 t 测算,对草履虫细胞存活数量的统计分析得到草履虫对应的存活时间(如图c)。
                        图b:zenCELL owl设置条件                                           图c:ImageJ和IBM® SPSS® Statistics v22.0处理原始图片后的存活曲线
 
       该文章,首次利用草履虫这一模式生物做为水质的生物指标,有效证实了混合气凝胶对重金属的原位吸附处理能力,为水质环境的改良提供了更严谨的理论基础。
 
       德国innoME公司自主研发和生产的zenCELL owl活细胞动态成像及分析系统,基于非标记、非侵入方法,原位记录细胞实时生长和定量的分析数据。zenCELL owl体积小巧,耐温耐湿,可在细胞培养箱内长时间连续工作;通过24个显微成像镜头,对24个视野进行连续的动态监测和图像获取。zenCELL owl为用户提供高质量图像、视频、统计学曲线和定量数据,实现细胞增殖/增殖抑制、细胞培养质控/培养优化、迁移/侵袭等诸多活细胞动态过程的监测。
 
       环亚生物,生命科学产品全国性代理商,不断把国外顶级的创新产品引入中国。环亚生物具备完善的公司运营、产品管理、营销、售前技术支持和售后维修体系。环亚生物作为zenCELL owl大中华区独家代理商,负责zenCELL owl产品大中华区的营销和售后支持。
 
       更多资讯请访问文献全文链接:
https://www.sciencedirect.com/science/article/pii/S0045653521004884?via%3Dihub
 

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