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上海交通大学系统生物医学研究院陶生策教授团队联合中科院武汉病毒研究所,佛山第四人民医院,中科院生物物理研究所等单位在著名期刊nature communications上在线发表新冠研究重要研究成果“SARS-CoV-2 proteome microarray for global profiling of COVID-19 specific IgG and IgM responses”。
 
该研究团队基于新冠病毒蛋白的基因编码序列,通过密码子优化,基因合成,病毒蛋白的体外表达和纯化获得病毒蛋白。然后通过Arrayjet Super Marathon 芯片点样仪将这些新冠病毒蛋白喷点到覆有硝酸纤维素膜的载玻片芯片上(Grace Bio-Labs,PATH 芯片),制备出新冠病毒蛋白组芯片。该芯片覆盖预测的28种新冠病毒蛋白中的18种。一张芯片上具有14个新冠病毒蛋白组微阵列,可进行14个样品的检测。

图1. 新冠病毒蛋白组芯片制作及实验流程

通过对新冠患者血清样本进行检测,作者发现所有参检患者血清均含有能与新冠病毒蛋白结合的IgG与IgM抗体, 尤其是特异性结合新冠病毒N蛋白与S1蛋白的抗体。另外,作者发现抗体还对新冠病毒蛋白ORF9b与NSP5表现出很强的反应。S1蛋白特异性IgG信号与患者年龄和乳酸脱氢酶(LDH)水平呈正相关并与淋巴细胞百分比成负相关。 
图2,b: 用新冠病毒蛋白组芯片检测恢复期新冠患者血清样本和健康人群对照血清样本;绿色和红色信号分别代表IgG和IgM的反应信号。  

该研究团队提供了新冠患者免疫系统IgG和IgM抗体的系统性研究,为开发有效的新冠病毒诊断,治疗和疫苗,提供了有价值的线索。
 
更多资讯请访问文献全文链接:https://www.nature.com/articles/s41467-020-17488-8
 
 
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